Immobilization of Organic Solvent-Tolerant Lipase from Pseudomonas mendocina M-37 with Potential Synthetic Activities
نویسندگان
چکیده
Praveen Dahiya, Subhash Chand and Neeraj Dilbaghi* Department of Bioand Nanotechnology, Guru Jambheshwar University of Science and Technology, Hisar-125001, Haryana, India Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India Present address: Amity Institute of Biotechnology, Amity University, Sector 125, Gautam Buddha Nagar, Noida-201303, Uttar Pradesh, India
منابع مشابه
Isolation, identification, biochemical properties and potential application of an organic solvent-tolerant lipase from Pseudomonas sp. strain NEB-1
Using enrichment procedures, 45 organic solvent-tolerant lipase producer bacterial strains were screened from areas contaminated by oil and organic solvents. Among the strains, 15 isolates exhibited extreme stability toward organic solvents and high lipolytic activity. The NEB-1 isolate which was later identified as Pseudomonas sp. strain NEB-1 by biochemical tests and 16S rDNA gene sequence an...
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An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 degrees C and 9.0, respectively. The purified lipase was stable at 45 degrees C and pH 6-9. It exhibited the highest stability in the presence of various org...
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An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids....
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BACKGROUND Whole-cell biocatalysis in organic solvents has been widely applied to industrial bioprocesses. In two-phase water-solvent processes, substrate conversion yields and volumetric productivities can be limited by the toxicity of solvents to host cells and by the low mass transfer rates of the substrates from the solvent phase to the whole-cell biocatalysts in water. RESULTS To solve t...
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